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81.
鸡(土从)连续发酵生产营养酱油,其最适接种量为20%,发酵时间7d;果胶酶和纤维素酶混合处理发酵液,可显著提高氨基态N和总N含量,氨基酸生成率达62.8%。 相似文献
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以甘蔗糖蜜为碳源发酵生产高生物量饲料酵母,通过单因素正交试验设计,确定优化的培养条件为:温度28℃,pH5.0,装液量200mL/500mL,摇床转速180r/min,培养时间84h。在优化的培养条件下酵母菌株的总蛋白质含量可达到6.01g/L。 相似文献
84.
灌注果寡糖对生长绵羊瘤胃发酵功能的影响 总被引:6,自引:0,他引:6
采用营养灌注技术研究了果寡糖对生长绵羊瘤胃发酵功能的影响。试验选取6只安装有永久性瘤胃瘘管的内蒙古半细毛羯羊,随机分为两组,试验组一灌注1.00%果寡糖,试验组二灌注2.00%果寡糖。结果表明:灌注果寡糖可以显著(P〈0.05)降低试羊的瘤胃液相pH和瘤胃内的NH3-N浓度,显著提高(P〈0.05)瘤胃内的VFA含量和MCP含量。两个灌注水平都可以提高生长绵羊的瘤胃发酵功能。但2.00%的灌注水平仅在瘤胃NH3-N浓度的降幅方面比1.00%的灌注水平显著增大(P〈0.05),在对瘤胃液相pH值、VFA含量和MCP含量的影响方面两个灌注水平没有显著差异(P〉0.05)。 相似文献
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Sanae ASANO Shoshichi IKEDA Yuzo KUROKAWA Shuhei KANDA Hisao ITABASHI 《Animal Science Journal》2007,78(1):28-33
To investigate seasonal variations in the digestive functions of sika deer, five female sika deer were provided with an amount of alfalfa hay cubes equivalent to voluntary food intake during winter. We measured the rate at which the food passed through the digestive tract, digestibility and rumen fermentation during the summer (August), autumn (November), winter (February) and spring (May). Total mean retention time in the digestive tract during summer and autumn was numerically longer than that in winter and spring, but the difference did not reach significance. Organic matter and fiber were less digestible in summer and autumn than in winter and spring (P < 0.05), whereas the digestibility of the dry matter tended to vary with the seasons (P < 0.1). Ruminal pH values seasonally changed (P < 0.01), and were the lowest in autumn. The concentration of ruminal ammonia‐nitrogen differed significantly among the seasons (P < 0.05), increasing in winter and decreasing during spring and summer. The numbers of protozoa changed significantly among the seasons (P < 0.05), being higher in autumn than in winter and spring, and intermediate in summer. The concentration of total volatile fatty acids was not seasonally affected, but the molar percentages of propionic acid and butyric acid significantly changed according to season (P < 0.05 and P < 0.01, respectively), and the ratio of acetic to propionic acid tended to change with the seasons (P < 0.1). The results of this study suggested that the digestive functions in sika deer, fed a commercial diet at a restricted level, differed notably among the seasons and these variations might partially be due to environmental effects. 相似文献
87.
Effects of amino nitrogen on fermentation parameters by mixed ruminal microbes when energy or nitrogen is limited 总被引:1,自引:0,他引:1
Hiroshi KAJIKAWA Kiyoshi TAJIMA Makoto MITSUMORI Akio TAKENAKA 《Animal Science Journal》2007,78(2):121-128
Ruminal microbes harvested from a ruminally fistulated cow were incubated in simple batch and semicontinuous cultures with NH3‐N or amino‐N on nitrogen‐ or energy‐excess diets in quantity (HN and LN diets, respectively, consisting of timothy hay plus soybean meal, or corn grain), based on evaluation with the National Research Council and Cornell Net Carbohydrate and Protein System models. In a batch culture experiment, supplementation with amino‐N promoted digestion and fermentation in the course of incubation (4–24 h) on both diets, but these effects mostly disappeared when the diets were sufficiently digested (at 48 h). In a semicontinuous culture experiment using Rusitec, no effect of amino‐N was exhibited after sufficient fermentation and digestion, but significant promotion of digestion was shown in the course of incubation on the HN diet, while no such effect was detected on the LN diet. The microbial yield for 24 h did not show a significant difference between the N sources of either of the two diets. These results suggest that the stimulatory effects of amino‐N are diminished when the diets are sufficiently digested after a long retention and incubation, and also that the effectiveness of amino‐N does not require a quantitatively energy‐excess status. 相似文献
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菊粉体外培养猪鲜粪对其微生物数量的影响 总被引:1,自引:0,他引:1
通过体外培养法探讨菊粉对猪鲜粪培养物pH和其中微生物数量的影响。试验设计0、1、2、4、6和8g/L6个菊粉水平分别进行体外厌氧培养生长猪鲜粪,24h后测定培养基pH、大肠杆菌、乳酸杆菌、双歧杆菌和总细菌数量的变化。结果表明,随着菊粉添加水平的增加,培养物pH和大肠杆菌数直线下降(P〈O.001),而乳酸杆菌、双歧杆菌以及总细菌数则直线上升(P〈0.01或P=0.059)。提示菊粉可促进猪鲜粪培养物中乳酸杆菌、双歧杆菌的增殖,抑制大肠杆菌的滋长,从而使总细菌数增加,pH下降,并具有剂量应答关系。 相似文献